Saturday, January 27, 2007

winter week 3

i got so much homework this weekend its crazy. and its only week 3.

Acting
- Contentless Scene Analysis and Write-Up (A “contentless scene” involves a sequence of dialogue attributed to no specific context. Contentless scene work enables students to apply the principles of scene construction. Students will learn that in a contentless scene it is necessary to create relationship, conflict, intention, obstacle and action.)
- Contentless Scene Rehearsal with Scene Partner
- Heart of a Dog Acting Critique

Public Speaking
- Preparation Outline and Speaking Outline for Speech to Inform wrt the Singapore Educational System
- Practice with Speech Partner (Facial Cues and Expression)
- Critique on Bush's State of the Union

Cell Biology
- Read tons of pages from Alberts' Molecular Biology of the Cell
- Review Session on Sunday
- Read papers assigned
- Midterm on Tuesday

Eukaryotic Gene Expression: Beyond the Central Dogma
- Read assigned paper: "RNA-mediated response to heat shock in
mammalian cells
" by Shamovsky et al (Nature 2006)
- Complete one page critique

Lab
- In Vitro Transcription of new pFK22 construct
- Purify and verify RNA
- Microinjections!

Help!

Sunday, January 21, 2007

Come What May... I Will Love You, Until My Dying Day

Never knew I could feel like this
Like I've never seen the sky before
Want to vanish inside your kiss
Everyday I love you more and more
Listen to my heart, can you hear it sing
Telling me to give you everything
Seasons may change, winter to spring
But I love you... until the end of time

Come what may, come what may
I will love you until my dying day

Suddenly the world seems such a perfect place
Suddenly it moves with such a perfect grace
Suddenly my life doesn't seem such a waste
It all revolves around you

And there's no mountain too high, no river too wide
Sing out this song and I'll be there by your side
Storm clouds may gather and stars may collide
But I love you until the end of time

Come what may, come what may
I will love you until my dying day
Oh come what may, come what may
I will love you

Suddenly the world seems such a perfect place...

Come what may, come what may
I will love you until my dying day

viaje del invierno en México (winter trip in Mexico) !

see how we've gotten lazier (and more adventurous!) over the years...

winter trip #1 - east coast USA:
a totally well-planned trip - cities to go, # of days at each city, a rough idea of what to do. booked all accommodations and read up a fair bit beforehand.

winter trip #2 - Italy:
a little less planning, most of the accommodations booked, leaving some leeway for negotiation. din read up too much, and had some impromptu exciting moments.

and the latest..

winter trip #3 - Mexico!
had a rough idea of which cities to cover (based entirely on a itinerary we found online for some backpacking tour), booked a one-way ticket into Mexico city. and off we went!

Review of trip: fun, excitement-packed, satisfying... and not to mention, good & cheap food, inexpensive in general, and more merry times with my Dearest!

a little note - although all the travel guides warn tourists to be extra careful in Mexico, where crime is prevalent, we din encounter any problems watsoever... so if u're thinking of going there, just take the necessary precautions, but nothing to be awfully worried about, we feel. =)


First, i MUST mention that the end of my semester was simply TOO exciting. i left sch on wed, less than half a week into the finals week! took my only exam on tuesday, packed and left early the next morning! and guess what, i actually still had a paper due that day at 5pm EST. so the paper-writing was almost entirely done on the flight (with amazingly high level of concentration!), finished on the drive back to the apartment, compiled and checked upon arrival in the house and submitted.... OMG... =P *grinz*

And the trip started off with a BIG BANG! hotel san francisco was booked thru priceline to ensure having a roof over our heads in the first few nites. turned out to be one of our most expensive accommodations actually... but certainly worth the $$, with the exciting surprise install for us that night!


the wonderful comfy hotel bed! and mexico, here we are!

so the story goes, we came back from dinner that night, and met this guy with a lady in the elevator who were going up. both were dressed well, heading up to the top floor. after an exchange of smiles, they said there was an employee party upstairs going on, and warmly invited us to join. "tequila, party, dance... come come!"

and so we went to take a look and 凑热闹 later that night. the music was playing loudly (we could even hear it from our room couple of floors down) with many pple on the dance floor doing the salsa... ooh la la! and so we were pulled into the dancing and drinking... i must say that the guys up there are superb at leading dances!! wat a night, wat a blasting first night in mexico!

**
of course, where else to find the authentic tacos and quesos but in mexico! but unlike here in the states, where the usual variety = beef/chicken/fish/veggie made in the same way, everywhere we went, the tacos and quesos were all different, different taste, meat... yum!


queso example #1


queso example #2 (this one's green-skinned!)


this was our first dinner... this beef soup thingy which was really yummy! too bad we never found it again elsewhere... (probably cos we din know it's name! haha)


so we had thought that the spray-painters in times square, new york were amazing... until we saw the even greater variety of paintings done by the painters in mexico! WOW...


with plenty of fruits sold all around in mexico, while darling was drooling over all the meat in the tacos, i was certainly kept satisfied with the many fruit cups we had! and mexicans have (almost) all their food their special way, including the fruits... topped with chilli powder, lime and a pinch of salt.


the view of the Palacio de Bellas Artes from the rooftop-viewing platform @ La Torre Latinoamericana, the highest building in mexico city.


the view of mexico city


looking down at the streets... an interesting fact of mexico city: at least one policeman stands at every junction of the downtown area, "beeeepp, beeeppp"-ing away at their whistles to direct traffic, disregarding the traffic lights!


it was one week before christmas, and the festive celebrations had long started, especially at the main city square!


a huge and pretty piñata in the city square. it is a seven-pointed star, representing the devil and the seven deadly sins. it is usually made of papier-mâché, so that it's easily breakable, for one to hit with a stick signifying the letting go of the good things that one has taken. the star is filled with candy and toys inside, which falls out when the star (devil) is broken.


pretty decorations! Feliz Navidad (merry christmas)...


and Feliz Ano (happy new year)!


HUGE sparklers to light up the night and join in the festivities!

Saturday, January 20, 2007

Thanksgiving @ Sequoia National Park

Ok, this is a little outdated, but still, better late than never, right? =) The gang finally got our act together and went somewhere over the long thanksgiving break late last november. here's a few selected shots with breaktaking views. =)
Sequoia trees are commonly thought to be the largest tree species out there: larger than whales, and NPS says one of them is THE "world's largest living thing"!
one of the more "normal"-sized sequoias for size comparison.
We rented a nice navy blue Dodge Durango SUV for the trip to fit all 6 of us. Roomy!
We bought so much food (~$100) that it filled up the entire bear-proof food cabinet. As bears are sensitive to smells and may attempt to seek out the items, we have to keep all food and stuff with strong smells (eg toiletries) locked in this metal cabinet that can only be opened with a special human-hand-only handle. Pictures posted around the campsite showed smashed car windows caused by bears foraging for human food. nasty!
setting up our two tents: a small two-men and a bigger four-men tent.
Here is Jason, the brains for the trip. Thanks for all the planning!
the firewood/charcoal we bought from the supermarket is really cool. each log is nicely and completely wrapped in a plastic foil. simply place the entire package into the pit and LIGHT THE PLASTIC FOIL at two points. the foil will catch fire, slowly spreading the flames throughout and lighting the log as well. look ma, clean hands! ingenious!
We attempted to kidnap home with two nice (and unbroken) pine cones as souvenirs. unfortunately, we left them on the food cabinet overnight and was caught by a roving ranger the next morning. we were "advised" that bringing anything out of the park is illegal, and he confiscated the two lonely pine cones from us. =(
We did not cover many trails as we all "chose" to sleep our mornings away and the sun sets pretty early. We did, however, cover the Big Baldy trail and came back with brilliant pictures! the sun was setting by the time we reach the end of the trail, which is a huge granite boulder outcrop (hence, the name "big baldy") that is supposed to give a nice view of the surrounding landscape. but since the sun was setting, the clouds was setting too, and we were treated to a cloudtop view. only bad part of this was driving back to the campsite down the winding roads in the midst of these setting clouds, otherwise known as fog. could hardly see 5 meters in front. jason was the best for doing the tough driving with only koklim to accompany him while the rest of us slept in the back. =P

the "view" from big baldy.
the gang @ big baldy.
our three fairies...
magnificient sunset.
and of course, my current desktop wallpaper, darlings above the clouds:

Sunday, January 14, 2007

I Fell for a Man Who Wore an Electronic Ankle Bracelet

Interesting Article on NYTimes today. Worth a read...

Modern Love
I Fell for a Man Who Wore an Electronic Ankle Bracelet
By ASHLEY CROSS
Published: January 14, 2007


FOR nearly three years I dated a guy who had been dismissed from Harvard over accusations of raping another student. I lived with him during a summer of his house arrest for his conviction on sexual assault charges and traveled to be with him during the school year.

Throughout, I strove to create a relationship of hopeful normalcy despite his electronic ankle bracelet, public ridicule and compromised future. Until finally, sadly, our affair ended, though not for reasons you might guess.

We had met at a summer camp where we were both counselors. He was 20, a swim instructor who caught my attention with his blue eyes, swollen lips and penchant for performing silly “Saturday Night Live” routines during the evening skits.

I was a 19-year-old equestrian recuperating from a traumatic break-up with my first love. As such, I wasn’t looking for a new relationship. But I found myself falling for him anyway, and soon we were inseparable, referred to by one annoyed counselor as “an endless J. Crew ad.”

Continue reading...

Monday, January 08, 2007

CAT 125 Essay - last part

This post is part of an on-going series on my CAT 125 project.
Check the introductory post here for more details.


Rise and Fall – The Journey of a Scientist
or where I see myself in 30 years time

(continuing from part 4)

Today, Bo_ne_ _all hides bashfully behind its dilapidated façade, leaving few traces of its glorious past. (Various letters had fallen off and stayed off since the beginning of last year.) The second and third floors are now virtually emptied of labs as scores of investigators retired, passed away or migrated to better facilities across the Atlantic or just across the road. Across the road is the state-of-the-art Natural Science Building (NSB), a glass-and-concrete, award-winning modernist building that houses scientists from biology, chemistry / biochemistry, and physics in shared facilities on each of the 6 floors. The lack of funding and modern equipment in Bonner severely hampers this old maiden’s ability to attract young faculty into her fold. Tellingly, the few new faculty to join UCSD are starting their labs in the sparkling new NSB.

And the story returns to the flies still wandering aimlessly inside the glass doors of Bonner Hall. (The flies from a year back are long dead, but this station at the frontiers of fly exploration will always be manned by fresh blood.) The Chinese has a saying that translates simply to “we are what we eat”. They believe that eating a pig’s brain allows us to “build up” our own and hence improve our intelligence. (Thereby explaining the great demand for ferocious tiger’s penis.) Perhaps, in a similar way, the idea can be translated to “we are what we do” – biologists are just like the flies we work on. We cultivate the flies, use them for a short time, pamper them, torture them, and finally chuck them out with the trash. Analogously, Science cultivates our passion, squeezes our brains for ideas for a few years, pampers us with fame, tortures us with experimental failures, and chucks us out into the real world when we run out of ideas to contribute to her growth.

But scientists are a unique breed, simply different from the normal man in the street. Born with an acute sense of our surroundings and a calling from bigger mysteries of life, we ignore temptations of higher pay and respect in other professions, ignore our need for a personal life outside work, and throw ourselves headlong, illogically perhaps, into this passion. Years of continuous 90 hour weeks, throughout and running well after the prime years of our lives, scientists work for pittance merely to generate decent results that may grace the cover of a (hopefully) respectable journal. And yet, the work accumulated after a life-long career is something hardly understood by the general public, much less appreciated. Science throws scientists Evolution cards – only the fittest survive and continue doing Science. Science is logical, non-emotional, exactly the right way how good science should be conducted. No name, no fame, no shame. Just as moths willingly fly straight into an open flame, gladly dying for their love and passion, scientists are “blinded” by our own belief of the supremacy of Science, crowding out each other to jump into this one-way train to destiny. This is a short journey of passion, of discovery, of bitter sweet success and agonizing failures. A short flash of inspiration and insight before we, like all others, are replaced with fresh blood. But nonetheless, hopefully, in the midst of all the hurry, a destiny of satisfaction, of strong belief, that Science will show us the right way forward, into a better future for us all.

*****

I now work on the 4th floor (south-west wing) of Bonner Hall, in a worm lab led by Dr A, a young, single female on her first term as an assistant professor. Dr A is a energetic damsel thriving in a male-dominated field, and has received multiple accolades in her budding career. A hard worker (though not yet a slave driver), she already has two major publications under her name from this lab, and has continuously been invited to present at major scientific meetings and to write reviews in major scientific journals. Her future looks bright, her journey just starting. I can’t help but wonder if, in the little free time she has, she has pondered about the careers of her colleagues, and how her own will mirror theirs.

Looking back, I realized that perhaps, I need not have fumbled my explanation to Prof Q with fictitious “truths” that I was too busy with school work and have no spare time for his lab. Prof Q, just like Prof J, Prof S, Prof F, Prof A and I are just members of a subspecies, servants for a cause, living through this life cycle that defines what a scientist is. They have been in my place twenty, thirty years ago. And I will be in their place twenty, thirty years from today. My experiences are hardly unique to myself, just like science is hardly unique to any one person.

I look forward to living their lives.

* the end *


Posts from the CAT 125 Series
- Written Abstract
- Visual Abstract
- Essay part 1
- Essay part 2
- Essay part 3
- Essay part 4
- Essay part 5 (end)

Thursday, January 04, 2007

CAT 125 Essay - part 4

This post is part of an on-going series on my CAT 125 project.
Check the introductory post here for more details.


Rise and Fall – The Journey of a Scientist
or where I see myself in 30 years time

(continuing from part 3)

Life sped by for the next few months. My first taste of real scientific research also opened my eyes to its more tacky side. Prof Q was not the first, nor was he the last to live the ultimate nightmare of a research scientist. In the long, tense relationship between science and federal funding, the amount of funding a scientist manages to secure regularly makes or breaks careers. Federal funding uses a peer review system to judge the quality of the proposal submitted, much like the system used to validate manuscripts before they are published in scientific journals and acknowledged as part of our organized body of knowledge. The peer review system is an anonymous-reviewer system where fellow researchers familiar with the area of research scrutinize proposals submitted to funding agencies for their quality, feasibility and effectiveness, before deciding which one among the thousands of proposals submitted receives a share of the limited funding. In this way, federal funding allocation is just like the science it funds – a completely objective, unbiased approach to gauge, to judge and then to act with no consideration of its impact on egos, fame or careers.

It was this principle that was applied to Prof J, a former Bonner Hall 3rd floor occupant who had moved on to head a big research institute on the UCSD Medical School campus. The grapevine told of two graduate students in her lab who were forced to graduate prematurely because she obtained only three out of the five grants she applied for. Their futures are now uncertain. Prof J was widely considered to be a highly successful female scientist. With no partner or family to saddle her down, she was able to focus her intelligence on solving research problems and micromanaging her lab. Her reputation for efficiency and enterprise had brought her up the career ladder, culminating in multiple high profile roles in the scientific community. But power and fame play no role in the objective allocation of federal research funding. Prof J, like Prof Q and everyone else, had to bite the dust and return to the drawing boards when their grant proposals fail to meet the grade, continuing to seek a better research question that their peers will deem worthy of funding.

*****

Prof F is a biochemistry professor who works on the 3rd floor of Bonner Hall. I had frequent interactions with him when I took an undergraduate tutor (TA) position over the summer for an undergraduate biology class he taught. A former Dean of the Biology Department and recipient of numerous teaching awards, Prof F is an unassuming, white-haired gentleman in his early sixties with eyes that shone with intelligence and a personality that radiates in spite of his soft-spoken words. In short, he fits the stereotypical image of a learned man through and through. Over coffee one morning (at a TA meeting), he unexpectedly announced, without a hint of sadness or regret, that he had lost his funding for the new year (gasp!), and promptly declared his plans for closing his lab, finishing the second edition of a textbook he authored, spending more time with his family, on teaching and with students and wondering where else he should devote his time and energies into. I was slightly surprised, but I soon remembered to close my jaw. Even as the group’s conversation burrowed deep into America’s involvement in Iraq and raged through the skirmish between Israel and Hezbollah in Lebanon, my mind was solely overwhelmed by this new perspective on research funding. In this case, the cut in funding is not the end, but in fact, a new beginning, a new lease of life.

A few weeks later, I found myself wandering around an empty lab, staring at the stained bench tops that had once been cluttered with chemical reagents, DNA samples, bacteria plates, and experimental protocols. My lab manager and I had come to scavenge Prof F’s lab for leftover lab supplies – reagents, glassware, radioactive shields, water baths etc. Everything that was slightly useful would be recycled or reused by the other (surviving) labs. I thought I would feel very sorry for Prof J, and wondered what it feels to have strangers and friends peck the flesh off your baby of 35 years. But I did not think much further than that, because I realized all my thinking was meaningless. By that time, I had understood.

(to be continued...)

Posts from the CAT 125 Series
- Written Abstract
- Visual Abstract
- Essay part 1
- Essay part 2
- Essay part 3
- Essay part 4
- Essay part 5 (end)